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CSNK2A2/CSNK2B (Human) Recombinant ProteinBioActive

  • Catalog # : P5527
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Human CSNK2A2 (NP_0011887.1, 1 a.a. - 350 a.a.) and CSNK2B (NP_001311.3, 1 a.a. - 215 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 68
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 97 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CKtide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5527
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 1459
  • Protein Accession#:
  • NP_001887.1 (Gene ID : 1459);NP_001311.3 (Gene ID : 1460)
  • Gene Name:
  • CSNK2A2
  • Gene Alias:
  • CK2A2,CSNK2A1,FLJ43934
  • Gene Description:
  • casein kinase 2, alpha prime polypeptide
  • Other Designations:
  • -
  • Gene Information
  • Entrez GeneID:
  • 1460
  • Protein Accession#:
  • NP_001887.1 (Gene ID : 1459);NP_001311.3 (Gene ID : 1460)
  • Gene Name:
  • CSNK2B
  • Gene Alias:
  • CK2B,CK2N,CSK2B,G5A,MGC138222,MGC138224
  • Gene Description:
  • casein kinase 2, beta polypeptide
  • Gene Summary:
  • This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq
  • Other Designations:
  • Casein kinase II beta subunit,OTTHUMP00000029060,OTTHUMP00000029063,OTTHUMP00000029064,alternative name: G5a, phosvitin,phosvitin
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