CSNK2A1/CSNK2B (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human CSNK2A1 (NP_001886.1, 1 a.a. - 391 a.a.) was coexpressed as N-terminal GST-fusion protein (72 kDa) and with human His-tagged CSNK2B (NP_001311.3, 1 a.a. - 215 a.a.) recombinant protein expressed in baculovirus system.
Sequence
GST-CSNK2A1: MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGAMGSMSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ
His-CSNK2B: MSYYHHHHHHDYDIPTTENLYFQGAMGSMSSSEEVSWISWFCGLRGNEFFCEVDEDYIQDKFNLTGLNEQVPHYRQALDMILDLEPDEELEDNPNQSDLIEQAAEMLYGLIHARYILTNRGIAQMLEKYQQGDFGYCPRVYCENQPMLPIGLSDIPGEAMVKLYCPKCMDVYTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKRPANQFVPRLYGFKIHPMAYQLQLQAASNFKSPVKTIRHost
insect
Form
Liquid
Preparation Method
Baculovirus expression system
Purification
Glutathione sepharose chromatography
Purity
98 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol).
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CSNK2A1
Entrez GeneID
1457Protein Accession#
NP_001886.1 (Gene ID : 1457);NP_001311.3 (Gene ID : 1460)Gene Name
CSNK2A1
Gene Alias
CK2A1, CKII
Gene Description
casein kinase 2, alpha 1 polypeptide
Omim ID
115440Gene Ontology
HyperlinkGene Summary
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq
Other Designations
CK2 catalytic subunit alpha|OTTHUMP00000029925|OTTHUMP00000029926|casein kinase II alpha 1 subunit|casein kinase II alpha subunit|protein kinase CK2
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Gene Info — CSNK2B
Entrez GeneID
1460Protein Accession#
NP_001886.1 (Gene ID : 1457);NP_001311.3 (Gene ID : 1460)Gene Name
CSNK2B
Gene Alias
CK2B, CK2N, CSK2B, G5A, MGC138222, MGC138224
Gene Description
casein kinase 2, beta polypeptide
Omim ID
115441Gene Ontology
HyperlinkGene Summary
This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq
Other Designations
Casein kinase II beta subunit|OTTHUMP00000029060|OTTHUMP00000029063|OTTHUMP00000029064|alternative name: G5a, phosvitin|phosvitin
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Interactome
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Pathway
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Disease
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