CSNK2A2/CSNK2B (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human CSNK2A2 (NP_0011887.1, 1 a.a. - 350 a.a.) and CSNK2B (NP_001311.3, 1 a.a. - 215 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
68
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
97 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CSNK2A2
Entrez GeneID
1459Protein Accession#
NP_001887.1 (Gene ID : 1459);NP_001311.3 (Gene ID : 1460)Gene Name
CSNK2A2
Gene Alias
CK2A2, CSNK2A1, FLJ43934
Gene Description
casein kinase 2, alpha prime polypeptide
Omim ID
115442Gene Ontology
HyperlinkGene Summary
O
Other Designations
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Gene Info — CSNK2B
Entrez GeneID
1460Protein Accession#
NP_001887.1 (Gene ID : 1459);NP_001311.3 (Gene ID : 1460)Gene Name
CSNK2B
Gene Alias
CK2B, CK2N, CSK2B, G5A, MGC138222, MGC138224
Gene Description
casein kinase 2, beta polypeptide
Omim ID
115441Gene Ontology
HyperlinkGene Summary
This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq
Other Designations
Casein kinase II beta subunit|OTTHUMP00000029060|OTTHUMP00000029063|OTTHUMP00000029064|alternative name: G5a, phosvitin|phosvitin
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Interactome
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Disease
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