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CAMKK1 (Human) Recombinant ProteinBioActive

  • Catalog # : P5507
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  • Specification
  • Product Description:
  • Human CAMKK1 (NP_115670.1, 1 a.a. - 505 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 82
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 72 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin . The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CAMKK substrate. ATP: 500 uM
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5507
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • CAMKK1
  • Gene Alias:
  • CAMKKA,DKFZp761M0423,MGC34095
  • Gene Description:
  • calcium/calmodulin-dependent protein kinase kinase 1, alpha
  • Gene Summary:
  • The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • CAMKK alpha protein,OTTHUMP00000115904,calcium/calmodulin-dependent protein kinase 1 alpha
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