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Deoxyribonuclease I (Bovine), Molecular Biology GradeBioActive

  • Catalog # : P5308
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  • Specification
  • Product Description:
  • Lyophilized. RNase & Protease free.
  • Host:
  • Bovine
  • Theoretical MW (kDa):
  • 31.3
  • Form:
  • Lyophilized
  • Preparation Method:
  • Native protein chromatographically purified from Bovine Pancreas
  • Activity:
  • >=2,000 Kunitz units per mg dry weight. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.
  • Recommend Usage:
  • Deoxyribonuclease I Assay; Nick translation; DNA mapping; Isolation of nuclear RNA and protein; Plasmid construction; RNA polymerase synthesis of RNA probes and RT-PCR.
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    Each vial contains approximately 2 mg glycine and 2 umoles calcium per 10,000 units of DNase I. Reconstituted the entire vial in 5 ml provides the equivalent of a 1 mg/ml solution.
  • Note:
  • Contains glycine and calcium as stabilizer. All equipment used to handle product should be RNase-free. Glassware and spatulas need to be heat-treated.
  • Biological function:
  • Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position.
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
  • Gene Information
  • Gene Name:
  • DNASE1
  • Gene Alias:
  • -
  • Gene Description:
  • deoxyribonuclease I
  • Other Designations:
  • -
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