DNASE1 Recombinant Protein
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Specification
Product Description
Lyophilized. Animal derived components free. RNases & proteases free. Recombinant protein expressed in Pichia pastoris.
Biological function
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position.
Host
Yeast
Theoretical MW (kDa)
31.3
Form
Lyophilized
Preparation Method
Yeast expression system
Activity
>=5000 units per mg protein. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.
Recommend Usage
Deoxyribonuclease I Assay; Removing genomic DNA from RNA preparations prior to RT-PCR; Degradation of DNA templates after transcription reactions; Removing unwanted DNA from samples prior to Northern blotting; Removing DNA during biopharma and bioprocessing procedures.
Storage Instruction
Store at 4°C on dry atmosphere.
Note
Contains glycine as a stabilizer. All equipment used to handle product should be RNase-free. Glassware and spatulas need to be heat-treated.
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Applications
Enzyme Activity
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Gene Info — DNASE1
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