Lyophilized. 0.22 um filtered. Material is not tested for pyrogenicity.
Theoretical MW (kDa):
Native protein purified from Bovine Pancreas
>=2,000 Kunitz units per mg dry weight. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.
Deoxyribonuclease I Assay; DNA removal in primary cell isolation to decreases viscosity providing better yields; DNA removal in bioprocessing applications.
Store at 4°C on dry atmosphere.
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position.