The activity is determined by the dose-dependent proliferation of mouse M-NFS-60 cells. The expected ED50 for this effect is 8-12 pg/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Storage Buffer:
No additive
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Serial dilutions of mouse Csf3, starting at 10 ng/mL, were added to NFS-60 cells. After 69 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50.