Csf3 (Mouse) Recombinant Protein
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Result of activity analysis
Result of activity analysis
Serial dilutions of mouse Csf3, starting at 10 ng/mL, were added to NFS-60 cells. After 69 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50.
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Specification
Product Description
Mouse Csf3 (P09920) recombinant protein expressed in Escherichia coli.
Sequence
MVPLVTVSALPPSLPLPRSFLLKSLEQVRKIQASGSVLLEQLCATYKLCHPEELVLLGHSLGIPKASLSGCSSQALQQTQCLSQLHSGLCLYQGLLQALSGISPALAPTLDLLQLDVANFATTIWQQMENLGVAPTVQPTQSAMPAFTSAFQRRAGGVLAISYLQGFLETARLALHHLA
Host
Escherichia coli
Theoretical MW (kDa)
19
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent proliferation of mouse M-NFS-60 cells. The expected ED50 for this effect is 8-12 pg/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Storage Buffer
No additive
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Csf3
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