Csf3 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Csf3 recombinant protein expressed in Escherichia coli.
Sequence
VPLVTVSALPPSLPLPRSFLLKSLEQVRKIQASGSVLLEQLCATYKLCHPEELVLLGHSLGIPKASLSGCSSQALQQTQCLSQLHSGLCLYQGLLQALSGISPALAPTLDLLQLDVANFATTIWQQMENLGVAPTVQPTQSAMPAFTSAFQRRAGGVLAISYLQGFLETARLALHHLA
Host
Escherichia coli
Theoretical MW (kDa)
18.9
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 98% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 0.05 ng/mL, measured by a cell proliferation assay using murine NFS-60 cells, corresponding to a specific activity of 2.0 x 107 IU/mg.
Storage Buffer
Lyophilized from a solution containing 10 mM NaCitrate, pH 4.0, 150 mM NaCl. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Csf3
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