PRDX5 (Human) Recombinant Protein
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Specification
Product Description
Human PRDX5 (NP_036226, 53 a.a. - 214 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
MAPIKVGDAIPAVEVFEGEPGNKVNLAELFKGKKGVLFGVPGAFTPGCSKTHLPGFVEQAEALKAKGVQVVACLSVNDAFVTGEWGRAHKAEGKVRLLADPTGAFGKETDLLLDDSLVSIFGNRRLKRFSMVVQDGIVKALNVEPDGTGLTCSLAPNIISQL
Host
Escherichia coli
Theoretical MW (kDa)
17
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
1 mg/mL
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Activity
Specific activity: >9,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1 ug of enzyme can reduce at 25°C for 1 minute.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM HEPES, pH 7.4.
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — PRDX5
Entrez GeneID
25824Protein Accession#
NP_036226Gene Name
PRDX5
Gene Alias
ACR1, AOEB166, B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10
Gene Description
peroxiredoxin 5
Omim ID
606583Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
Alu co-repressor 1|TPx type VI|antioxidant enzyme B166|liver tissue 2D-page spot 71B|peroxisomal antioxidant enzyme|thioredoxin peroxidase PMP20
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Interactome
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Disease
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