< 1.0 EU per 1 microgram of protein (determined by LAL method)
Specific activity: approximately 117-136 pmole/min/ug. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX5 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide
Quality Control Testing:
Loading 3 ug protein in 15% SDS-PAGE
In 20 mM HEPES, pH 7.4
Store at 4°C for 1-2 weeks. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Alu co-repressor 1,TPx type VI,antioxidant enzyme B166,liver tissue 2D-page spot 71B,peroxisomal antioxidant enzyme,thioredoxin peroxidase PMP20