RFP tag monoclonal antibody, clone RF5R
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Specifications
Product Description
Mouse monoclonal antibody raised against synthetic peptide of RFP tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of sea anemon RFP tag.
Host
Mouse
Specificity
Recognizes native and denatured forms of RFP and its variants: TagRFP, TurboRFP, DsRed, mCherry, mOrange, tdTomato, etc.
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Western Blot (1:1000-1:3000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis probed against HEK293 cells transfected with RFP-tagged protein vector; untransfected control (1), transfected with Turbo-RFP (2), and transfected with DeRed (3). Blotting with RFP tag monoclonal antibody, clone RF5R (Cat # MAB9754) at 1:1000 dilution.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot
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Publication Reference
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The brain vascular damage-induced lymphatic ingrowth is directed by Cxcl12b/Cxcr4a.
Jingying Chen, Jianbo He, Lingfei Luo.
Development 2022 Jul; 149(13):dev200729.
Application:ISH, Zebrafish, Zebrafish larvae.
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Tel2 regulates redifferentiation of bipotential progenitor cells via Hhex during zebrafish liver regeneration.
Junren Zhang, Yang Zhou, Shuang Li, Dashuang Mo, Jianlong Ma, Rui Ni, Qifen Yang, Jianbo He, Lingfei Luo.
Cell Reports 2022 Apr; 39(1):110596.
Application:ISH, RFP protein.
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The brain vascular damage-induced lymphatic ingrowth is directed by Cxcl12b/Cxcr4a.
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