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Last updated: 2016/12/4
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TUBB2B monoclonal antibody, clone AT5B3

  • Catalog # : MAB9717
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant TUBB2B.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-445 of human TUBB2b.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • ELISA (1:250)
    Western Blot (1:250-1:500)
    Immunofluorescence (1:250-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of cell lysates (35 ug) with TUBB2B monoclonal antibody, clone AT5B3 (Cat # MAB9717) at 1 : 500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
    Lane 1 : HeLa.
    Lane 2 : 293T.
    Lasne 3 : mouse brain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with TUBB2B monoclonal antibody, clone AT5B3 (Cat # MAB9717) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of human SW480 cells stained with TUBB2B monoclonal antibody, clone AT5B3 (Cat # MAB9717) at 1 : 250 dilution with Texas Red (Red).
    Arrow head : Mitotic cell.
    Arrow : MTOC (microtubule organizing center).
  • ELISA
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • TUBB2B
  • Gene Alias:
  • DKFZp566F223,FLJ98847,MGC8685,TUBB-PARALOG,bA506K6.1
  • Gene Description:
  • tubulin, beta 2B
  • Other Designations:
  • OTTHUMP00000015964,class II beta-tubulin isotype,tubulin, beta polypeptide paralog
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