Mouse monoclonal antibody raised against partial recombinant TUBB2B.
Immunogen:
Recombinant protein corresponding to amino acids 1-445 of human TUBB2b.
Host:
Mouse
Reactivity:
Human, Mouse
Form:
Liquid
Purification:
Protein G purification
Concentration:
1 mg/mL
Isotype:
IgG2a, kappa
Recommend Usage:
ELISA Flow Cytometry Immunohistochemistry Immunocytochemistry Immunofluorescence Western Blot The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with TUBB2B monoclonal antibody, clone AT5B3 (Cat # MAB9717) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).