TUBB2B monoclonal antibody, clone AT5B3
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with TUBB2B monoclonal antibody, clone AT5B3 (Cat # MAB9717) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant TUBB2B.
Immunogen
Recombinant protein corresponding to amino acids 1-445 of human TUBB2b.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with TUBB2B monoclonal antibody, clone AT5B3 (Cat # MAB9717) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Enzyme-linked Immunoabsorbent Assay
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Gene Info — TUBB2B
Entrez GeneID
347733Protein Accession#
NP_821080Gene Name
TUBB2B
Gene Alias
DKFZp566F223, FLJ98847, MGC8685, TUBB-PARALOG, bA506K6.1
Gene Description
tubulin, beta 2B
Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000015964|class II beta-tubulin isotype|tubulin, beta polypeptide paralog
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Interactome
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Pathway
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