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Applications
Western Blot (Cell lysate)
Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with KCTD15 monoclonal antibody, clone AT4C3 (Cat # MAB8482) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: KCTD15 Transfected 293T cell lysate.
Immunocytochemistry
Immunofluorescence
Immunofluorescence of human HeLa cells stained with KCTD15 monoclonal antibody, clone AT4C3 (Cat # MAB8482) (1 : 500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).