Mouse monoclonal antibody raised against full length recombinant SNCA.
Immunogen:
Recombinant protein corresponding to full length human SNCA.
Host:
Mouse
Theoretical MW (kDa):
15
Reactivity:
Human, Rat
Specificity:
This antibody has been directly tested for reactivity in human and rodent. It is expected that it will work on other mammal tissues. specific to the ~15KDa alpha Synuclein protein.
Form:
Liquid
Isotype:
IgG
Recommend Usage:
Western Blot (1:1000) Immunofluorescence (1:500) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (10 mM sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
alpha-Synuclein locus triplication causes Parkinson's disease. Singleton AB, Farrer M, Johnson J, Singleton A, Hague S, Kachergus J, Hulihan M, Peuralinna T, Dutra A, Nussbaum R, Lincoln S, Crawley A, Hanson M, Maraganore D, Adler C, Cookson MR, Muenter M, Baptista M, Miller D, Blancato J, Hardy J, Gwinn-Hardy K.Science. 2003 Oct 31;302(5646):841.
Western blot of rat brain lysate showing specific immunolabeling ~ 15 kDa of SNCA protein. Using SNCA monoclonal antibody (Cat # MAB8432).
Immunofluorescence
Mixed hyman neuron-glial cultures stained with SNCA (red) and chicken MAP2 (green). The SNCA monoclonal antibody (Cat # MAB8432) stains vesicular structures, the perikarya and processes of the neurons in this image.
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq
Other Designations:
OTTHUMP00000161559,OTTHUMP00000161561,alpha synuclein,alpha-synuclein,alpha-synuclein, isoform NACP140,non A-beta component of AD amyloid,non A4 component of amyloid