NPM1 monoclonal antibody, clone 7H10B9
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More Files
- More Functions
Western Blot (Cell lysate)
Wetern blot analysis of SMMC-7721 cell extracts with NPM1 monoclonal antibody, clone 7H10B9 (Cat # MAB8313).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis pf paraffin-embedded human liver carcinoma, showing nuclear localization using NPM1 monoclonal antibody, clone 7H10B9 (Cat # MAB8313) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant NPM1.
Immunogen
Recombinant protein corresponding to human NPM1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:500-1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:2000)
Immunohistochemistry (Frozen sections) (1:500-1:2000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Wetern blot analysis of SMMC-7721 cell extracts with NPM1 monoclonal antibody, clone 7H10B9 (Cat # MAB8313).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis pf paraffin-embedded human liver carcinoma, showing nuclear localization using NPM1 monoclonal antibody, clone 7H10B9 (Cat # MAB8313) with DAB staining.Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Gene Info — NPM1
Entrez GeneID
4869Gene Name
NPM1
Gene Alias
B23, MGC104254, NPM
Gene Description
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Gene Ontology
HyperlinkGene Summary
NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM
Other Designations
nucleolar phosphoprotein B23|nucleophosmin 1|nucleophosmin/nucleoplasmin family, member 1|numatrin
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Interactome
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Disease
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Publication Reference
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The t(3;5)(q25.1;q34) of myelodysplastic syndrome and acute myeloid leukemia produces a novel fusion gene, NPM-MLF1.
Yoneda-Kato N, Look AT, Kirstein MN, Valentine MB, Raimondi SC, Cohen KJ, Carroll AJ, Morris SW.
Oncogene 1996 Jan; 12(2):265.
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Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma.
Morris SW, Kirstein MN, Valentine MB, Dittmer KG, Shapiro DN, Saltman DL, Look AT.
Science 1994 Mar; 263(5151):1281.
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The t(3;5)(q25.1;q34) of myelodysplastic syndrome and acute myeloid leukemia produces a novel fusion gene, NPM-MLF1.
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