NPM1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
NPM1 polyclonal antibody (Cat # PAB6762) (0.1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NPM1.
Immunogen
A synthetic peptide corresponding to human NPM1.
Sequence
C-QEAIQDLWQWRKSL
Host
Goat
Theoretical MW (kDa)
32.6, 29.5
Reactivity
Human, Mouse, Rat
Specificity
This antibody is expected to recognize both report isoforms (NP_002511.1, NP_954654.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NPM1 polyclonal antibody (Cat # PAB6762) (0.1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NPM1
Entrez GeneID
4869Protein Accession#
NP_002511.1;NP_954654.1Gene Name
NPM1
Gene Alias
B23, MGC104254, NPM
Gene Description
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Gene Ontology
HyperlinkGene Summary
NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM
Other Designations
nucleolar phosphoprotein B23|nucleophosmin 1|nucleophosmin/nucleoplasmin family, member 1|numatrin
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Interactome
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Disease
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Publication Reference
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NPM mutations in acute myelogenous leukemia.
Grisendi S, Pandolfi PP.
The New England Journal of Medicine 2005 Jan; 352(3):291.
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NPM mutations in acute myelogenous leukemia.
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