Mouse monoclonal antibody raised against partial recombinant TNNI3.
Recombinant protein corresponding to truncated human TNNI3.
Western Blot (1:200-1:1000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:1000) Immunohistochemistry (Frozen sections) (1:200-1:1000) ELISA (1:10000) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human normal cardiac muscle tissue showing cytoplasmic localization, using TNNI3 monoclonal antibody, clone 3A10A12, 5C3A7 (Cat # MAB8299) with DAB staining.
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq
familial hypertrophic cardiomyopathy 7,troponin I, cardiac