RAF1 monoclonal antibody, clone M208
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of RAF1.
Immunogen
A synthetic peptide corresponding to N-terminus of human RAF1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This C-Raf sequence has high homology to rat and mouse C-Raf.
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of human A-431 (Lane 1) and Jurkat (Lane 2) cells probed with RAF1 monoclonal antibody, clone M208 (Cat # MAB6771) at 1 : 500.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAF1
Entrez GeneID
5894Gene Name
RAF1
Gene Alias
CRAF, NS5, Raf-1, c-Raf
Gene Description
v-raf-1 murine leukemia viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq
Other Designations
Oncogene RAF1|raf proto-oncogene serine/threonine protein kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Nuclear localized Raf1 isoform alters DNA-dependent protein kinase activity and the DNA damage response.
Nixon BR, Sebag SC, Glennon MS, Hall EJ, Kounlavong ES, Freeman ML, Becker JR.
FASEB Journal 2018 Aug; fj201800336R.
Application:WB-Ce, Human, HEK cells.
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Nuclear localized Raf1 isoform alters DNA-dependent protein kinase activity and the DNA damage response.
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