RAF1 recombinant monoclonal antibody, clone R05-9B1
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More Files
- More Functions
Western Blot
Western blot analysis of SFRS3 in Jurkat, C6, Hela lysates using SFRS3 antibody.
Western Blot
Western blot analysis of Raf1 in C6, 3T3, Hela lysates using Raf1 antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RAF1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human RAF1.
Theoretical MW (kDa)
Calculated MW: 73 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of SFRS3 in Jurkat, C6, Hela lysates using SFRS3 antibody.Western Blot
Western blot analysis of Raf1 in C6, 3T3, Hela lysates using Raf1 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
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Gene Info — RAF1
Entrez GeneID
5894Protein Accession#
P04049Gene Name
RAF1
Gene Alias
CRAF, NS5, Raf-1, c-Raf
Gene Description
v-raf-1 murine leukemia viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq
Other Designations
Oncogene RAF1|raf proto-oncogene serine/threonine protein kinase
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Interactome
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Pathway
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Disease
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