Thy1 monoclonal antibody, clone G7 (SPRD)
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Specifications
Product Description
Rat monoclonal antibody raised against Thy1.
Immunogen
MST cell hybridoma C6/G8.
Host
Rat
Reactivity
Mouse
Specificity
Specificity Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A)
Form
Liquid
Conjugation
SPRD
Isotype
IgG2c, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Flow Cytometry
BALB/c mesenteric lymph node cells were stained with either rat IgG2c-FITC (as a negative control) or Thy1 monoclonal antibody, clone G7 (FITC) (Cat # MAB5953). Lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Thy1
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Publication Reference
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Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism.
Hueber AO, Raposo G, Pierres M, He HT.
The Journal of Experimental Medicine 1994 Mar; 179(3):785.
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Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death.
Ucker DS, Meyers J, Obermiller PS.
Journal of Immunology 1992 Sep; 149(5):1583.
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Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism.
He HT, Naquet P, Caillol D, Pierres M.
The Journal of Experimental Medicine 1991 Feb; 173(2):515.
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Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism.
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