Thy1 monoclonal antibody, clone 30-H12 (APC)
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More Files
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Flow Cytometry
BALB/c splenocytes were double-stained with Thy1 monoclonal antibody, clone 30-H12 (APC) (Cat # MAB5958) and Cd19 monoclonal antibody, clone 6D5 (PE) (Cat # MAB5689). Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against native Thy1.
Immunogen
Native purified Thy1 from mouse spleen cells or thymocyte membranes.
Host
Rat
Reactivity
Mouse
Specificity
Specificity Mouse CD90.2/Thy-1.2 alloantigen, Mr 18 KDa
Form
Liquid
Conjugation
APC
Isotype
IgG2b, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Flow Cytometry
BALB/c splenocytes were double-stained with Thy1 monoclonal antibody, clone 30-H12 (APC) (Cat # MAB5958) and Cd19 monoclonal antibody, clone 6D5 (PE) (Cat # MAB5689). Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Thy1
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Publication Reference
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Complete structure of the glycosyl phosphatidylinositol membrane anchor of rat brain Thy-1 glycoprotein.
Homans SW, Ferguson MA, Dwek RA, Rademacher TW, Anand R, Williams AF.
Nature 1988 May; 333(6170):269.
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Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin.
Williams AF, Gagnon J.
Science 1982 May; 216(4547):696.
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T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views.
Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA.
The Journal of Experimental Medicine 1980 Aug; 152(2):280.
Application：IF, IHC-Fr, Flow Cyt, Mouse, Mouse T cells, Mouse lymph node.
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Complete structure of the glycosyl phosphatidylinositol membrane anchor of rat brain Thy-1 glycoprotein.
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