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Last updated: 2021/1/17
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MYL2 monoclonal antibody, clone AT3B2 

  • Catalog # : MAB5641
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant MYL2.
  • Immunogen:
  • Recombinant protein corresponding to full length human MYL2.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of mouse heart tissue lysate.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of A431 cells. The cell was stained with MYL2 monoclonal antibody, clone AT3B2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of A431 cells. The cell was stained with MYL2 monoclonal antibody, clone AT3B2 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 4633
  • Gene Name:
  • MYL2
  • Gene Alias:
  • CMH10,DKFZp779C0562,MLC2
  • Gene Description:
  • myosin, light chain 2, regulatory, cardiac, slow
  • Gene Summary:
  • Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq
  • Other Designations:
  • RLC of myosin,cardiac ventricular myosin light chain 2,myosin light chain 2,myosin regulatory light chain 2, ventricular/cardiac muscle isoform,myosin, light polypeptide 2, regulatory, cardiac, slow,regulatory light chain of myosin,slow cardiac myosin reg
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