MYL2 (phospho Y118) polyclonal antibody
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More Files
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Immunofluorescence
Immunofluorescent staining of methanol-fixed U87 cells using MYL2 (phospho Y118) polyclonal antibody (Cat# PAB29624) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MYL2.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y118 of human MYL2.
Host
Rabbit
Theoretical MW (kDa)
20
Reactivity
Human
Specificity
MYL2 (phospho Y118) polyclonal antibody detects endogenous level of MYL2 only when phosphorylated at tyrosine 118.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of methanol-fixed U87 cells using MYL2 (phospho Y118) polyclonal antibody (Cat# PAB29624) at 1:100-1:200 dilution. -
Gene Info — MYL2
Entrez GeneID
4633Protein Accession#
P10916Gene Name
MYL2
Gene Alias
CMH10, DKFZp779C0562, MLC2
Gene Description
myosin, light chain 2, regulatory, cardiac, slow
Gene Ontology
HyperlinkGene Summary
Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq
Other Designations
RLC of myosin|cardiac ventricular myosin light chain 2|myosin light chain 2|myosin regulatory light chain 2, ventricular/cardiac muscle isoform|myosin, light polypeptide 2, regulatory, cardiac, slow|regulatory light chain of myosin|slow cardiac myosin reg
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Interactome
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Pathway
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Disease
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