MYL2 monoclonal antibody, clone AT3B2
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse heart tissue lysate.
Immunofluorescence
Immunofluorescence analysis of A431 cells. The cell was stained with MYL2 monoclonal antibody, clone AT3B2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometry analysis of A431 cells. The cell was stained with MYL2 monoclonal antibody, clone AT3B2 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant MYL2.
Immunogen
Recombinant protein corresponding to full length human MYL2.
Host
Mouse
Reactivity
Human, Rat
Form
Liquid
Purification
Protein A purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse heart tissue lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of A431 cells. The cell was stained with MYL2 monoclonal antibody, clone AT3B2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of A431 cells. The cell was stained with MYL2 monoclonal antibody, clone AT3B2 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). -
Gene Info — MYL2
Entrez GeneID
4633Protein Accession#
NP_000423Gene Name
MYL2
Gene Alias
CMH10, DKFZp779C0562, MLC2
Gene Description
myosin, light chain 2, regulatory, cardiac, slow
Gene Ontology
HyperlinkGene Summary
Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq
Other Designations
RLC of myosin|cardiac ventricular myosin light chain 2|myosin light chain 2|myosin regulatory light chain 2, ventricular/cardiac muscle isoform|myosin, light polypeptide 2, regulatory, cardiac, slow|regulatory light chain of myosin|slow cardiac myosin reg
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Interactome
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Pathway
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Disease
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Publication Reference
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Mutations in either the essential or regulatory light chains of myosin are associated with a rare myopathy in human heart and skeletal muscle.
Poetter K, Jiang H, Hassanzadeh S, Master SR, Chang A, Dalakas MC, Rayment I, Sellers JR, Fananapazir L, Epstein ND.
Nature Genetics 1996 May; 13(1):63.
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Localization of the gene coding for ventricular myosin regulatory light chain (MYL2) to human chromosome 12q23-q24.3.
Macera MJ, Szabo P, Wadgaonkar R, Siddiqui MA, Verma RS.
Genomics 1992 Jul; 13(3):829.
Application：IF, IHC, WB-Ce, WB-Tr, Human, Mammalian cells.
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Mutations in either the essential or regulatory light chains of myosin are associated with a rare myopathy in human heart and skeletal muscle.
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