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Last updated: 2018/7/22
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NEFH monoclonal antibody, clone AH1

  • Catalog # : MAB5386
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against NEFH.
  • Immunogen:
  • Bovine NEFH.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 200-220
  • Reactivity:
  • Bovine
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Western Blot (1:10000)
    Immunofluorescence (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (5 mM sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Strip blots of crude rat spinal cord extract stained with NEFH monoclonal antibody, clone AH1 (Cat # MAB5386).
    All three antibodies bind to a prominent band with an apparent SDS-PAGE molecular weight of 200 KDa.
  • Immunohistochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Mixed neuron/glial cultures stained with NEFH monoclonal antibody, clone AH1 (Cat # MAB5386) (green) and also stained with rabbit neurofilament NF-L polyclonal antibody (red).
    The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NEFH monoclonal antibody, clone AH1 (Cat # MAB5386) binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments.
    In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated NF-H they appear golden in color.
    Blue shows the distribution of DNA.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry
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