NEFH monoclonal antibody, clone AH1
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More Files
- More Functions
Western Blot (Tissue lysate)
Strip blots of crude rat spinal cord extract stained with NEFH monoclonal antibody, clone AH1 (Cat # MAB5386).
All three antibodies bind to a prominent band with an apparent SDS-PAGE molecular weight of 200 KDa.
Immunofluorescence
Mixed neuron/glial cultures stained with NEFH monoclonal antibody, clone AH1 (Cat # MAB5386) (green) and also stained with rabbit neurofilament NF-L polyclonal antibody (red).
The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NEFH monoclonal antibody, clone AH1 (Cat # MAB5386) binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments.
In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated NF-H they appear golden in color.
Blue shows the distribution of DNA.
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Specification
Product Description
Mouse monoclonal antibody raised against NEFH.
Immunogen
Bovine NEFH.
Host
Mouse
Theoretical MW (kDa)
200-220
Reactivity
Bovine
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:10000)
Immunofluorescence (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (5 mM sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Strip blots of crude rat spinal cord extract stained with NEFH monoclonal antibody, clone AH1 (Cat # MAB5386).
All three antibodies bind to a prominent band with an apparent SDS-PAGE molecular weight of 200 KDa.Immunohistochemistry
Immunofluorescence
Mixed neuron/glial cultures stained with NEFH monoclonal antibody, clone AH1 (Cat # MAB5386) (green) and also stained with rabbit neurofilament NF-L polyclonal antibody (red).
The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NEFH monoclonal antibody, clone AH1 (Cat # MAB5386) binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments.
In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated NF-H they appear golden in color.
Blue shows the distribution of DNA.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H.
Harris J, Ayyub C, Shaw G.
Journal of Neuroscience Research 1991 Sep; 30(1):47.
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A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H.
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