Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01 (Cat # MAB3826). IgM heavy chain and IgM light chain (25-30 KDa) indicated. MW of alpha tubulin is around 50 KDa. L = lysate. IPr = immunoprecipitate (reducing conditions). IPn = immunoprecipitate (non-reducing conditions).
Western Blot (Cell lysate)
Use of alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826) as a loading control (A) in an Western blotting experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B).