Tubulin-alpha monoclonal antibody, clone TU-01
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More Files
- More Functions
Western Blot (Cell lysate)
Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01 (Cat # MAB3826).
IgM heavy chain and IgM light chain (25-30 KDa) indicated.
MW of alpha tubulin is around 50 KDa.
L = lysate. IPr = immunoprecipitate (reducing conditions). IPn = immunoprecipitate (non-reducing conditions).
Western Blot (Cell lysate)
Use of alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826) as a loading control (A) in an Western blotting experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B).
Immunofluorescence
Panel A : Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; green) and VIM monoclonal antibody, clone VI-01 (Cat # MAB3880 ; red). Nucleus is stained with DAPI (blue).
Panel B : Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; green). Nucleus is stained with DAPI (blue).
Immunofluorescence
Immunofluorescence staining of HeLa (human cervix carcinoma cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; red). Nucleus is stained with DAPI (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against native Tubulin-alpha.
Immunogen
Native purified Tubulin-alpha from porcine brain alpha tubulin (two cycles of polymerization-depolymerization).
Host
Mouse
Reactivity
Pig
Specificity
This antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
Form
Liquid
Isotype
IgG1
Recommend Usage
Flow Cytometry (1-4 ug/mL)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01 (Cat # MAB3826).
IgM heavy chain and IgM light chain (25-30 KDa) indicated.
MW of alpha tubulin is around 50 KDa.
L = lysate. IPr = immunoprecipitate (reducing conditions). IPn = immunoprecipitate (non-reducing conditions).Western Blot (Cell lysate)
Use of alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826) as a loading control (A) in an Western blotting experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Panel A : Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; green) and VIM monoclonal antibody, clone VI-01 (Cat # MAB3880 ; red). Nucleus is stained with DAPI (blue).
Panel B : Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; green). Nucleus is stained with DAPI (blue).Immunofluorescence
Immunofluorescence staining of HeLa (human cervix carcinoma cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; red). Nucleus is stained with DAPI (blue). -
Publication Reference
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Dazap2 modulates transcription driven by the Wnt effector TCF-4.
Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V.
Nucleic Acids Research 2009 Mar; 37(9):3007.
Application：WB-Tr, Human, Mouse, HEK 293 cells, Mouse C57MG.
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Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells.
Kukharskyy V, Sulimenko V, Macurek L, Sulimenko T, Draberova E, Draber P.
Experimental Cell Research 2004 Aug; 298(1):218.
Application：WB-Ce, Mouse , P19.X1 cells.
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Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells.
Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P.
Planta 1997 Jan; 201(3):349.
Application：IF, WB-Ce, Plant, Nicotiana tabacum L. cells.
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Dazap2 modulates transcription driven by the Wnt effector TCF-4.
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