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Tubulin-alpha monoclonal antibody, clone TU-01

  • Catalog # : MAB3826
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native Tubulin-alpha.
  • Immunogen:
  • Native purified Tubulin-alpha from porcine brain alpha tubulin (two cycles of polymerization-depolymerization).
  • Host:
  • Mouse
  • Reactivity:
  • Pig
  • Specificity:
  • This antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01 (Cat # MAB3826).
    IgM heavy chain and IgM light chain (25-30 KDa) indicated.
    MW of alpha tubulin is around 50 KDa.
    L = lysate. IPr = immunoprecipitate (reducing conditions). IPn = immunoprecipitate (non-reducing conditions).
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Use of alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826) as a loading control (A) in an Western blotting experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of HeLa (human cervix carcinoma cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; red). Nucleus is stained with DAPI (blue).
  • Immunofluorescence
  • Immunofluorescence
  • Panel A : Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; green) and VIM monoclonal antibody, clone VI-01 (Cat # MAB3880 ; red). Nucleus is stained with DAPI (blue).
    Panel B : Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; green). Nucleus is stained with DAPI (blue).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
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