INPP5D monoclonal antibody, clone SHIP-02
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Specifications
Product Description
Mouse monoclonal antibody raised against synthetic peptide of INPP5D.
Immunogen
A synthetic peptide corresponding to N-terminus human INPP5D.
Host
Mouse
Theoretical MW (kDa)
110, 125, 130, 135,
Reactivity
Human
Specificity
This antibody reacts with SHIP-1, a phosphoinositide phosphatase largely confinedto hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 KDa.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Flow Cytometry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting analysis (reducing conditions) of human INPP5D in whole cell lysate of THP-1 (human acute monocytic leukemia cell line).
Lane 1 : Immunostaining with Isotype mouse IgG1 control.
Lane 2, 3 : Immunostaining with INPP5D monoclonal antibody, clone SHIP-01 (Cat # MAB3645) Lane 4, 5 : Immunostaining with INPP5D monoclonal antibody, clone SHIP-02 (Cat # MAB3646).Flow Cytometry
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Gene Info — INPP5D
Entrez GeneID
3635Gene Name
INPP5D
Gene Alias
MGC104855, MGC142140, MGC142142, SHIP, SHIP1, SIP-145, hp51CN
Gene Description
inositol polyphosphate-5-phosphatase, 145kDa
Omim ID
601582Gene Ontology
HyperlinkGene Summary
This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. Overall, the protein functions as a negative regulator of myeliod cell proliferation and survival. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
SH2 containing inositol phosphatase|SH2 containing inositol phosphatase, isoform b|p150Ship|signaling inositol polyphosphate 5 phosphatase SIP-145
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs.
Rovenska M, Hlouchova K, Sacha P, Mlcochova P, Horak V, Zamecnik J, Barinka C, Konvalinka J.
Prostate 2008 Feb; 68(2):171.
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Expression of glutamate carboxypeptidase II in human brain.
Sácha P, Zámecník J, Barinka C, Hlouchová K, Vícha A, Mlcochová P, Hilgert I, Eckschlager T, Konvalinka J.
Neuroscience 2007 Feb; 144(4):1361.
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Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer.
Mesters JR, Barinka C, Li W, Tsukamoto T, Majer P, Slusher BS, Konvalinka J, Hilgenfeld R.
The EMBO Journal 2006 Mar; 25(6):1375.
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Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs.
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