Phosphoserine monoclonal antibody, clone 2A4
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More Files
- More Functions
Western Blot (Cell lysate)
The cell lysate derived from EGF-stimulated A-431was resolved onto 12% SDS-PAGE and immunoblotted by Phosphoserine monoclonal antibody, clone 2A4 (Cat # MAB2545) at 1 : 500 (Lane A) ; or pre-incubated by Phosphoserine (land B) or pre-incubated by phosphotyrosine (lnae C). A panel of phosphorylated proteins was observed.
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Specification
Product Description
Mouse monoclonal antibody raised against Phosphoserine.
Immunogen
A chemically linked phosphoserine.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes phosphoserine only. It does not cross react with phosphotyrosine or phosphothreonine.
Form
Liquid
Isotype
IgG
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (antibody stabilizer).
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from EGF-stimulated A-431was resolved onto 12% SDS-PAGE and immunoblotted by Phosphoserine monoclonal antibody, clone 2A4 (Cat # MAB2545) at 1 : 500 (Lane A) ; or pre-incubated by Phosphoserine (land B) or pre-incubated by phosphotyrosine (lnae C). A panel of phosphorylated proteins was observed.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Autoantibodies in primary Sjogren's syndrome patients induce internalization of muscarinic type 3 receptors.
Jin M, Hwang SM, Davies AJ, Shin Y, Bae JS, Lee JH, Lee EB, Song YW, Park K.
Biochimica et Biophysica Acta 2012 Feb; 1822(2):161.
Application：IP, Human, hSMG cells.
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Autoantibodies in primary Sjogren's syndrome patients induce internalization of muscarinic type 3 receptors.
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