Mouse monoclonal antibody raised against Phosphoserine.
A chemically linked phosphoserine.
Human, Mouse, Rat
This antibody recognizes phosphoserine only. It does not cross react with phosphotyrosine or phosphothreonine.
Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user.
In TBS, pH 7.2 (BSA, 10% Proclin300)
Store at 4°C. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months.
The cell lysate derived from EGF-stimulated A-431was resolved onto 12% SDS-PAGE and immunoblotted by Phosphoserine monoclonal antibody, clone A8G9 (Cat # MAB2545) at 1 : 500 (Lane A) ; or pre-incubated by Phosphoserine (land B) or pre-incubated by phosphotyrosine (lnae C). A panel of phosphorylated proteins was observed.