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Last updated: 2022/5/22

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H2AFX (acetyl K9) monoclonal antibody, clone RM446 

  • Catalog # : MAB23231
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic acetyl peptide of human H2AFX (acetyl K9).
  • Immunogen:
  • A synthetic acetyl peptide corresponding to residues surrounding K9 of H2AFX.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to H2AFX acetylated at Lysine 9 (K9ac). No cross reactivity with non-modified Lysine 9 or other acetylated Lysines in histone H2A.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot (0.01 ug/mL-0.1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: H2A recombinant protein, Lane 2: acid extracts of HeLa cell lysate using H2AFX (acetyl K9) monoclonal antibody, clone RM446 (Cat # MAB23231) under 0.01 ug/mL working concentration.
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 3014
  • Gene Name:
  • H2AFX
  • Gene Alias:
  • H2A.X,H2A/X,H2AX
  • Gene Description:
  • H2A histone family, member X
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
  • Other Designations:
  • H2AX histone
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