H2AX (phospho S139) recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1: HepG2 cell lysate–treated with etoposide Lane2: HepG2 cell lysate–untreated with H2AX (phospho S139) recombinant monoclonal antibody (Cat # RAB02688) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using H2AX (phospho S139) recombinant monoclonal antibody (Cat # RAB02688). Counter stained with hematoxylin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against H2AX.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant H2AX.
Theoretical MW (kDa)
15
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1: HepG2 cell lysate–treated with etoposide Lane2: HepG2 cell lysate–untreated with H2AX (phospho S139) recombinant monoclonal antibody (Cat # RAB02688) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using H2AX (phospho S139) recombinant monoclonal antibody (Cat # RAB02688). Counter stained with hematoxylin. -
Gene Info — H2AFX
Entrez GeneID
3014Protein Accession#
P16104Gene Name
H2AFX
Gene Alias
H2A.X, H2A/X, H2AX
Gene Description
H2A histone family, member X
Omim ID
601772Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
Other Designations
H2AX histone
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Interactome
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Pathway
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Disease
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