Histone H3 (dimethyl K9) and Histone H3 (acetyl K14) monoclonal antibody, clone RM322
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HeLa cells, Lane 2: recombinant histone H3.3 using Histone H3 (dimethyl K9) and Histone H3 (acetyl K14) monoclonal antibody, clone RM322 (Cat # MAB23157) under 0.5 ug/mL working concentration.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Histone H3 (dimethyl K9) and Histone H3 (acetyl K14) monoclonal antibody, clone RM322 (Cat# MAB23157) specifically reacts to Histone H3 only when modified at both dimethyl-lysine 9 and acetyl-lysine 14 (K9me2/K14ac). No cross reactivity with non- modified Lysine 9/14, methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, acetylated Lysine 9/14 ONLY, or methylated Lysine 14 (K14me2) ONLY, in Histone H3.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic dimethyl peptide corresponding to residues surrounding Lys9 of human histone H3 and a synthetic acetyl peptide corresponding to residues surrounding Lys14 of human histone H3.
Reactivity
Human
Specificity
This antibody reacts to histone H3 only when modified by both methylation at lysine 9 and acetylation at lysine 14. No cross reactivity with monomethylated Lysine 9, dimethylated Lysine 9, trimethylated Lysine 9 , or acetylated Lysine 9/14 only, in histone H3.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Western Blot (0.1 ug/mL-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HeLa cells, Lane 2: recombinant histone H3.3 using Histone H3 (dimethyl K9) and Histone H3 (acetyl K14) monoclonal antibody, clone RM322 (Cat # MAB23157) under 0.5 ug/mL working concentration.
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