Mouse monoclonal antibody raised against Rituximab. Target gene is CD20.
Immunogen:
Rituximab.
Host:
Mouse
Specificity:
The product is specific for Rituximab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Rituximab monoclonal antibody, clone 6C1 (Cat # MAB23136).
Form:
Lyophilized
Preparation Method:
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Rituximab.
Purification:
Protein A purification
Isotype:
IgG1
Recommend Usage:
ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect ELISA) Sandwich ELISA The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction:
Store at -20°C on dry atmosphere, lyophilized antibodies are stable at 1 years. After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Anti-Rituximab monoclonal antibody, clone 17B6 (Cat # MAB23137) binds with Rituximab. Coating antigen: Rituximab, 1 ug/mL. Anti-Rituximab monoclonal antibody, clone 17B6 (Cat # MAB23137) dilution start from 2000 ng/mL. EC50 = 5.075 ng/mL. While the antibody does not recognize the human IgG (data not shown).
Sandwich ELISA
Standard curve of Rituximab Sandwich ELISA. The Rituximab Sandwich ELISA assay is developed by using Anti-Rituximab monoclonal antibody, clone 6C1 (Cat # MAB23136) and Anti-Rituximab monoclonal antibody, clone 17B6 (Cat # MAB23137) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Rituximab monoclonal antibody, clone 17B6 (Cat # MAB23137) was labeled with Biotin. The sensitivity is less than 1 ng/mL.
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq