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Last updated: 2021/4/11

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Anti-Infliximab monoclonal antibody, clone 27A4 

  • Catalog # : MAB23110
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Infliximab.
  • Immunogen:
  • Infliximab.
  • Host:
  • Mouse
  • Specificity:
  • The product is specific for Infliximab. The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Infliximab monoclonal antibody, clone 23H5 (Cat # MAB23111).
  • Form:
  • Lyophilized
  • Preparation Method:
  • This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Infliximab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA (0.01-0.1 ug/mL (Direct/Indirect))
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • Non-inhibitory
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA binding of Anti-Infliximab monoclonal antibody, clone 27A4 (Cat # MAB23110) with Infliximab. Coating antigen: Infliximab, 1 ug/mL. Anti-Infliximab monoclonal antibody, clone 27A4 dilution start from 1000 ng/mL. EC50= 16.17 ng/mL. While the antibody does not recognize the human IgG (data not shown).
  • Sandwich ELISA
  • Sandwich ELISA
  • The Sandwich ELISA assay is developed by using Anti-Infliximab monoclonal antibody, clone 27A4 (Cat # MAB23110) and Anti-Infliximab monoclonal antibody, clone 23H5 (Cat # MAB23111) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Infliximab monoclonal antibody, clone 23H5 was labeled with Biotin. The sensitivity of detecting Infliximab is up to 0.195 ng/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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