Rabbit monoclonal antibody raised against Atezolizumab.
Immunogen:
Atezolizumab.
Host:
Rabbit
Specificity:
The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Atezolizumab monoclonal antibody, clone 55D8 (Cat # MAB23079).
Form:
Lyophilized
Conjugation:
Biotin
Preparation Method:
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Atezolizumab.
Purification:
Protein A purification
Isotype:
IgG
Recommend Usage:
ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect ELISA) Sandwich ELISA The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4 (1% BSA, 0.02% sodium azide).
Storage Instruction:
Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt. After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ELISA binding of Anti-Atezolizumab monoclonal antibody, clone 43H11 (Biotin) (Cat # MAB23081) with Atezolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Atezolizumab, 1 ug/mL. Anti-Atezolizumab monoclonal antibody, clone 43H11 (Biotin) (Cat # MAB23081) dilutions start from 1000 ng/mL. EC50 = 3.749 ng/mL.
Sandwich ELISA
Standard curve of Atezolizumab Sandwich ELISA. The Atezolizumab Sandwich ELISA assay is developed by using Anti-Atezolizumab monoclonal antibody, clone 55D8 (Cat # MAB23079) and Anti-Atezolizumab monoclonal antibody, clone 43H11 (Biotin) (Cat # MAB23081) as the capture and detection antibodies, respectively. The sensitivity of detecting Atezolizumab is up to 156 pg/mL.