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Last updated: 2021/4/11

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Anti-Atezolizumab monoclonal antibody, clone 55D8 

  • Catalog # : MAB23079
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against Atezolizumab.
  • Immunogen:
  • Atezolizumab.
  • Host:
  • Rabbit
  • Specificity:
  • The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080).
  • Form:
  • Lyophilized
  • Preparation Method:
  • This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Atezolizumab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (ELISA capture: 0.01-0.1 ug/mL; ELISA blocking: 10-50 ug/mL) (Direct/Indirect/Inhibitory ELISA)
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • 0.391 ug/mL
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA binding of Anti-Atezolizumab monoclonal antibody, clone 55D8 (Cat # MAB23079) with Atezolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Atezolizumab, 1 ug/mL. Anti-Atezolizumab monoclonal antibody, clone 55D8 (Cat # MAB23079) dilutions start from 1000 ng/mL. EC50 = 2.446 ng/mL.
  • Sandwich ELISA
  • Sandwich ELISA
  • Standard curve of Atezolizumab Sandwich ELISA. The Atezolizumab Sandwich ELISA assay is developed by using Anti-Atezolizumab monoclonal antibody, clone 55D8 (Cat # MAB23079) and Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Atezolizumab monoclonal antibody, clone 43H11 (Cat # MAB23080) was labeled with Biotin. The sensitivity of detecting Atezolizumab is up to 156 pg/mL.
  • Blocking
  • Blocking
  • Anti-Atezolizumab monoclonal antibody, clone 55D8 (Cat # MAB23079) blocks Atezolizumab binding with Human PD-L1 recombinant protein (PD-L1 Fc Chimera, Human). Coating antigen: Atezolizumab, 1 ug/mL. PD-L1-Fc-biotin final concentration: 10 ng/mL. Anti-Atezolizumab monoclonal antibody, clone 55D8 (Cat # MAB23079) dilutions start from 10 ug/mL. IC50 = 0.39 ug/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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