Rabbit monoclonal antibody raised against Adalimumab.
Immunogen:
Adalimumab.
Host:
Rabbit
Specificity:
The product is specific for Adalimumab. This antibody can't block Adalimumab binding with TNF-alpha (TNF-alpha, Human) in ELISA.
Form:
Lyophilized
Preparation Method:
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Adalimumab.
Purification:
Protein A purification
Isotype:
IgG
Recommend Usage:
ELISA (ELISA detection: 0.07-0.1 ug/mL) (Direct ELISA) Immunogenicity assay The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction:
Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt. After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ELISA binding of Anti-Adalimumab monoclonal antibody, clone 196F3 (Cat # MAB23075) with Adalimumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Adalimumab, 1 ug/mL. Anti-Adalimumab monoclonal antibody, clone 196F3 (Cat # MAB23075) dilution start from 10000 ng/mL. EC50 = 60.85 ng/mL.