LMNB2 monoclonal antibody, clone ADBF-12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate; Lane 2: NIH/3T3 cell lysate; Lane 3: C6 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human spleen.
Immunofluorescence
Immunofluorescent analysis of Hela cells.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human LMNB2.
Immunogen
A synthetic peptide corresponding to human LMNB2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
The antibody reacts with human, mouse, rat LMNB2, in native form and recombinant. Superfamily members of LMNB2 are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate; Lane 2: NIH/3T3 cell lysate; Lane 3: C6 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human spleen.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of Hela cells.Immunoprecipitation
Flow Cytometry
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Gene Info — LMNB2
Entrez GeneID
84823Protein Accession#
Q03252Gene Name
LMNB2
Gene Alias
LAMB2, LMN2, MGC2721
Gene Description
lamin B2
Gene Ontology
HyperlinkGene Summary
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq
Other Designations
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Interactome
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Disease
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