LMNB2 recombinant monoclonal antibody, clone R02-2J2
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human LMNB2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human LMNB2.
Theoretical MW (kDa)
Calculated MW: 70 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: C6, Lane 2: 3T3 and Lane 3: Hela lysates with LMNB2 recombinant monoclonal antibody, clone R02-2J2 (Cat # RAB02141).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human lung cancer with LMNB2 recombinant monoclonal antibody, clone R02-2J2 (Cat # RAB02141). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunofluorescence
Immunoprecipitation
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Gene Info — LMNB2
Entrez GeneID
84823Protein Accession#
Q03252Gene Name
LMNB2
Gene Alias
LAMB2, LMN2, MGC2721
Gene Description
lamin B2
Gene Ontology
HyperlinkGene Summary
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq
Other Designations
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Interactome
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Disease
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