RBBP4 monoclonal antibody, clone AEII-18
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More Files
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Specifications
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human RBBP4.
Immunogen
A synthetic peptide corresponding to human RBBP4.
Host
Rabbit
Reactivity
Human
Specificity
The antibody reacts with human RBBP4, in native form and recombinant. Superfamily members of RBBP4 are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysate.Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — RBBP4
Entrez GeneID
5928Protein Accession#
Q09028Gene Name
RBBP4
Gene Alias
NURF55, RBAP48
Gene Description
retinoblastoma binding protein 4
Omim ID
602923Gene Ontology
HyperlinkGene Summary
This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
MSI1 protein homolog|OTTHUMP00000009691|chromatin assembly factor/CAF-1 p48 subunit|retinoblastoma-binding protein 4|retinoblastoma-binding protein p48
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Interactomes
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Diseases
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