SMARCA5 monoclonal antibody, clone AFDB-19
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human SMARCA5.
Immunogen
A synthetic peptide corresponding to human SMARCA5.
Host
Rabbit
Reactivity
Human
Specificity
The antibody reacts with human SMARCA5, in native form and recombinant. Superfamily members of SMARCA5 are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MCF-7 cell lysate.Immunocytochemistry
Immunofluorescence
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Gene Info — SMARCA5
Entrez GeneID
8467Protein Accession#
O60264Gene Name
SMARCA5
Gene Alias
ISWI, SNF2H, WCRF135, hISWI, hSNF2H
Gene Description
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Omim ID
603375Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq
Other Designations
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a5|sucrose nonfermenting-like 5
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