N6-Methyladenosine monoclonal antibody, clone RM362
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against N6-Methyladenosine.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against BSA-conjugated N6-Methyladenosine.
Reactivity
Human
Specificity
This antibody reacts to N6-Methyladenosine (m6A) in both RNA and DNA. No cross reactivity with non-methylated adenosine.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Dot Blot (0.5 ug/mL-2 ug/mL)
ELISA (0.1 ug/mL-3 ug/mL)
MeRIP (0.2 ug/mL-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot of Lambda DNA without or with m6A.RIP Assay
MeRIP was performed using M6A and control RNA from NEB (E1610).
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