ATG4A monoclonal antibody, clone 8E8G2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ATG4A-hIgGFc transfected HEK293 cell lysates with ATG4A monoclonal antibody, clone 8E8G2 (Cat # MAB21322).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ATG4A monoclonal antibody, clone 8E8G2 (Cat # MAB21322).
Flow Cytometry
Flow cytometric analysis of K562 cells with ATG4A monoclonal antibody, clone 8E8G2 (Cat # MAB21322) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ATG4A.
Immunogen
Recombinant protein corresponding to amino acids 258-398 of human ATG4A.
Host
Mouse
Theoretical MW (kDa)
45.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ATG4A-hIgGFc transfected HEK293 cell lysates with ATG4A monoclonal antibody, clone 8E8G2 (Cat # MAB21322).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ATG4A monoclonal antibody, clone 8E8G2 (Cat # MAB21322).Flow Cytometry
Flow cytometric analysis of K562 cells with ATG4A monoclonal antibody, clone 8E8G2 (Cat # MAB21322) (Green). Red: Negative Control. -
Gene Info — ATG4A
Entrez GeneID
115201Protein Accession#
Q8WYN0Gene Name
ATG4A
Gene Alias
APG4A, AUTL2
Gene Description
ATG4 autophagy related 4 homolog A (S. cerevisiae)
Omim ID
300663Gene Ontology
HyperlinkGene Summary
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq
Other Designations
APG4 autophagy 4 homolog A|AUT-like 2, cysteine endopeptidase|OTTHUMP00000023822|OTTHUMP00000062893|autophagin 2|autophagy-related cysteine endopeptidase 2
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Interactome
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Pathway
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Disease
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