PDPN monoclonal antibody, clone 5E2
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunofluorescence
Immunofluorescence analysis of HaLe cells. The cell was stained with PDPN monoclonal antibody, clone 5E2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of HeLa cells. The cell was stained with PDPN monoclonal antibody, clone 5E2 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PDPN.
Immunogen
Recombinant protein corresponding to amino acids 1-206 of human PDPN.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HaLe cells. The cell was stained with PDPN monoclonal antibody, clone 5E2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells. The cell was stained with PDPN monoclonal antibody, clone 5E2 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). -
Gene Info — PDPN
Entrez GeneID
10630Protein Accession#
NP_006465Gene Name
PDPN
Gene Alias
AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2
Gene Description
podoplanin
Omim ID
608863Gene Ontology
HyperlinkGene Summary
This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000009640|OTTHUMP00000044504|glycoprotein, 36-KD|hT1alpha-1|hT1alpha-2|lung type I cell membrane associated glycoprotein|lung type-I cell membrane-associated glycoprotein|lung type-I cell membrane-associated glycoprotein (T1A-2)
-
Interactome
-
Disease
-
Publication Reference
-
The potential role of podoplanin in tumour invasion.
Wicki A, Christofori G.
British Journal of Cancer 2007 Jan; 96(1):1.
Application：IHC, Human, Human colon cancer.
-
Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium.
Zimmer G, Oeffner F, Von Messling V, Tschernig T, Groness HJ, Klenk HD, Herrler G.
The Biochemical Journal 1999 Jul; 341(Pt 2):277.
Application：IF, Human, Placental.
-
The potential role of podoplanin in tumour invasion.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com