Western blot analysis of Lane 1: MDA-MB-21 cell lysate, Lane 2: HeLa cell lysate, Lane 3: A431 cell lysate, Lane 4: NIH3T3 cell lysate, Lane 5: 293T cell lysate, Lane 6: HepG2 cell lysate.
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: Recombinant Human YWHAZ, Lane 2: Recombinant Human YWHAB, Lane 3: Recombinant Human YWHAE, Lane 4: Recombinant Human YWHAH, Lane 5: Recombinant Human YWHAG, Lane 6: Recombinant Human SFN, Lane 7: Recombinant Human YWHAQ.
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with YWHAQ monoclonal antibody, clone AT1A1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq
Other Designations:
14-3-3 protein T-cell,14-3-3 protein tau,14-3-3 protein theta,OTTHUMP00000015730,protein tau,tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide