PMS2 monoclonal antibody, clone 1204
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More Files
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Western Blot (Cell lysate)
Western blot analysis is shown using PMS2 monoclonal antibody, clone 1204 (Cat # MAB2089) to detect human PMS2 protein present in H157 cell lysates.
Approximately 5, 10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer.
A single band of ~96 KDa corresponding to the expected molecular weight for human PMS2 protein.
Personal communication Morphotek Inc.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PMS2.
Immunogen
Recombinant protein corresponding to the first 133 amino acids of human PMS2.
Host
Mouse
Reactivity
Chimpanzee, Human, Mouse, Rat
Specificity
This is an protein A purified antibody directed against PMS2-134 and reacts with full length version of PMS2 in human and hamster tissues. The epitope was putatively mapped to amino acids 58-81 of human PMS2.
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis is shown using PMS2 monoclonal antibody, clone 1204 (Cat # MAB2089) to detect human PMS2 protein present in H157 cell lysates.
Approximately 5, 10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer.
A single band of ~96 KDa corresponding to the expected molecular weight for human PMS2 protein.
Personal communication Morphotek Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PMS2
Entrez GeneID
5395Protein Accession#
P54278;NP_000526Gene Name
PMS2
Gene Alias
HNPCC4, PMS2CL, PMSL2
Gene Description
PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq
Other Designations
DNA mismatch repair protein|H_DJ0042M02.9|PMS2 postmeiotic segregation increased 2|mismatch repair endonuclease|postmeiotic segregation increased 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Mismatch repair gene PMS2: disease-causing germline mutations are frequent in patients whose tumors stain negative for PMS2 protein, but paralogous genes obscure mutation detection and interpretation.
Nakagawa H, Lockman JC, Frankel WL, Hampel H, Steenblock K, Burgart LJ, Thibodeau SN, de la Chapelle A.
Cancer Research 2004 Jul; 64(14):4721.
Application：IHC-P, WB-Ce, Human, Mouse , Human colon cancer, Human lymphoblastoid cells, Mouse-Human hybrid cells.
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ATM-mediated stabilization of hMutL DNA mismatch repair proteins augments p53 activation during DNA damage.
Luo Y, Lin FT, Lin WC.
Molecular and Cellular Biology 2004 Jul; 24(14):6430.
Application：WB, Human, H460, HCT-116, HEK 293, HFF cells, Human AT22IJE-T fibroblasts.
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Microsatellite instability, immunohistochemistry, and additional PMS2 staining in suspected hereditary nonpolyposis colorectal cancer.
de Jong AE, van Puijenbroek M, Hendriks Y, Tops C, Wijnen J, Ausems MG, Meijers-Heijboer H, Wagner A, van Os TA, Brocker-Vriends AH, Vasen HF, Morreau H.
Clinical Cancer Research 2004 Feb; 10(3):972.
Application：IHC-P, Human, Human ereditary nonpolyposis colorectal carcinoma.
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Hereditary nonpolyposis colorectal cancer and related conditions.
Lucci-Cordisco E, Zito I, Gensini F, Genuardi M.
American Journal of Medical Genetics. Part A 2003 Nov; 122A(4):325.
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Mismatch repair gene PMS2: disease-causing germline mutations are frequent in patients whose tumors stain negative for PMS2 protein, but paralogous genes obscure mutation detection and interpretation.
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