KLF4 monoclonal antibody, clone AT4E6
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: recombinant human KLF4 protein.
Flow Cytometry
Flow cytometric analysis of A549 cells. The cell was stained with KLF4 monoclonal antibody, clone AT4E6 at 2-5 ug for 1x106cells. (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KLF4.
Immunogen
Recombinant protein corresponding to amino acids 1-170 of human KLF4.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: recombinant human KLF4 protein.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of A549 cells. The cell was stained with KLF4 monoclonal antibody, clone AT4E6 at 2-5 ug for 1x106cells. (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). -
Gene Info — KLF4
Entrez GeneID
9314Protein Accession#
NP_004226.3Gene Name
KLF4
Gene Alias
EZF, GKLF
Gene Description
Kruppel-like factor 4 (gut)
Omim ID
602253Gene Ontology
HyperlinkOther Designations
OTTHUMP00000021862|endothelial Kruppel-like zinc finger protein
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Interactome
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Disease
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Publication Reference
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Platelet factor 4 mediates vascular smooth muscle cell injury responses.
Shi G, Field DJ, Long X, Mickelsen D, Ko KA, Ture S, Korshunov VA, Miano JM, Morrell CN.
Blood 2013 May; 121(21):4417.
Application：WB-Ce, WB-Tr, Human, HCASMCs.
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KLF4: a novel target for the treatment of atherosclerosis.
Yan FF, Liu YF, Liu Y, Zhao YX.
Medical Hypotheses 2008 Sep; 70(4):845.
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Genetic and epigenetic analysis of the KLF4 gene in gastric cancer.
Cho YG, Song JH, Kim CJ, Nam SW, Yoo NJ, Lee JY, Park WS.
APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica 2007 Jul; 115(7):802.
Application：IHC, Human, Human gastric cancer.
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Platelet factor 4 mediates vascular smooth muscle cell injury responses.
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