Western blot analysis of Lane 1: recombinant human KLF4 protein.
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of A549 cells. The cell was stained with KLF4 monoclonal antibody, clone AT4E6 at 2-5 ug for 1x106cells. (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).