KLF4 recombinant monoclonal antibody, clone 3A1
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: 293T whole cell lysate; Lane 3: PC-3 whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: A549 whole cell lysate; Lane 6: U87 whole cell lysate; Lane 7: Brain tissue.
Immunohistochemistry
Immunohistochemistry image of KLF4 recombinant monoclonal antibody, clone 3A1 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of Hela Cells with KLF4 recombinant monoclonal antibody, clone 3A1 at 1:50, counter-stained with DAPI.
Immunoprecipitation
Immunoprecipitating KLF4 in Hela whole cell lysate.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human KLF4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human KLF4.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation(1:200-1:1000)
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: 293T whole cell lysate; Lane 3: PC-3 whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: A549 whole cell lysate; Lane 6: U87 whole cell lysate; Lane 7: Brain tissue.Immunohistochemistry
Immunohistochemistry image of KLF4 recombinant monoclonal antibody, clone 3A1 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of Hela Cells with KLF4 recombinant monoclonal antibody, clone 3A1 at 1:50, counter-stained with DAPI.Immunoprecipitation
Immunoprecipitating KLF4 in Hela whole cell lysate.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KLF4
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Interactome
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Disease
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