RAB7A monoclonal antibody, clone GHA-18
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of (1) A375 cell lysate; (2) 3T3 cell lysate using RAB7A monoclonal antibody, clone GHA-18.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human RAB7A.
Immunogen
A synthetic peptide corresponding to human RAB7A.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) A375 cell lysate; (2) 3T3 cell lysate using RAB7A monoclonal antibody, clone GHA-18.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — RAB7A
Entrez GeneID
7879Protein Accession#
P51149Gene Name
RAB7A
Gene Alias
FLJ20819, PRO2706, RAB7
Gene Description
RAB7A, member RAS oncogene family
Gene Ontology
HyperlinkGene Summary
RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq
Other Designations
RAB7, member RAS oncogene family|Ras-associated protein RAB7
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Interactome
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Disease
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