POLR2A (phospho S5) monoclonal antibody, clone ABCE-16
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of (1) HeLa cell lysate treated with Lambda Phosphatase, (2) HeLa cell lysate using POLR2A (phospho S5) monoclonal antibody, clone ABCE-16.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human POLR2A.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S5 of human POLR2A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:40)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) HeLa cell lysate treated with Lambda Phosphatase, (2) HeLa cell lysate using POLR2A (phospho S5) monoclonal antibody, clone ABCE-16.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — POLR2A
Entrez GeneID
5430Protein Accession#
P24928Gene Name
POLR2A
Gene Alias
MGC75453, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
Gene Description
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Omim ID
180660Gene Ontology
HyperlinkGene Summary
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq
Other Designations
DNA directed RNA polymerase II polypeptide A|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|polymerase (RNA) II (DNA directed) polypeptide A (220kD)
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Interactome
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Pathway
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Disease
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