POLR2A (phospho S5) recombinant monoclonal antibody, clone 2H4
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: A549 whole cell lysate and Lane 3: 293 whole cell lysate withPOLR2A (phospho S5) recombinant monoclonal antibody, clone 2H4 (Cat # RAB04286).
Immunocytochemistry
Immunocytochemical staining of HeLa cells with POLR2A (phospho S5) recombinant monoclonal antibody, clone 2H4 (Cat # RAB04286) (diluated at 1:75).
Immunofluorescence
Immunofluorescent staining of Hela cells with POLR2A (phospho S5) recombinant monoclonal antibody, clone 2H4 (Cat # RAB04286) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human POLR2A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S5 of human POLR2A.
Theoretical MW (kDa)
Calculated MW: 270 k
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence(1:20-1:200)
Immunohistochemistry(1:50-1:200)
Western Blot(1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: A549 whole cell lysate and Lane 3: 293 whole cell lysate withPOLR2A (phospho S5) recombinant monoclonal antibody, clone 2H4 (Cat # RAB04286).Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of HeLa cells with POLR2A (phospho S5) recombinant monoclonal antibody, clone 2H4 (Cat # RAB04286) (diluated at 1:75).Immunofluorescence
Immunofluorescent staining of Hela cells with POLR2A (phospho S5) recombinant monoclonal antibody, clone 2H4 (Cat # RAB04286) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — POLR2A
Entrez GeneID
5430Protein Accession#
P24928Gene Name
POLR2A
Gene Alias
MGC75453, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
Gene Description
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Omim ID
180660Gene Ontology
HyperlinkGene Summary
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq
Other Designations
DNA directed RNA polymerase II polypeptide A|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|polymerase (RNA) II (DNA directed) polypeptide A (220kD)
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Interactome
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Pathway
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Disease
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